Glycan Processing

Glycosylation, the post-translational modification of a protein, lipid, or nucleic acid with specific carbohydrate chains, is a widespread phenomenon in biology. The two most common forms are N– and O-glycosylation, and the glycans can have significant effects on the properties of the biomolecule. Analysis of glycans is typically done through glycan removal or trimming using endo and/or exo-glycosidases, followed by downstream assays.

Lectenz Bio offers a suite of glycan processing enzymes. These include enzymes PNGase F, PNGase F-II, α2,3 Sialidase, and O-GlcNAcase.

Glycan Processing Enzymes

Product Name Catalog Number Amount Glycoprotein? Optimal pH Activity
PNGase F GE01014,000 units No 7.5-8.5 Cleaves N-linked glycans without core α1,3 fucose
PNGase F-II GE0201100 units No 6.5-7.5 Cleaves N-linked glycans with or without core fucose, both α1,6 and α1,3
α2,3 Sialidase GE0302 5,000 units No 7.0-7.5 Cleaves terminal α2,3-linked sialic acid
O-GlcNAcase GE0401 1,000 units No 7.5 Cleaves O-linked β-N-Acetylglucosamine (GlcNAc)
FasTEVTM Protease GE0501 1,000 units No 6.5-8.0 Cleaves the peptide bond between a glutamine and a glycine or serine of the consensus sequence ENLYFQ▲G/S
Thermophilic Fucosidase GE0601 5,000 units No 7.0-7.5 Cleaves α1,2/3/4/6-linked fucose at high temperature
Pan Sialidase GE0701 5,000 units No 7.0-7.5 Cleaves terminal sialic acid
Endo S GE0801 2,500 units No 5.5-6.5 Cleaves N-linked glycans from IgG heavy chain
β1,3 Galactosidase GE0901 2,000 units No 7.5-8.0 Cleaves terminal β1,3-linked galactose
α1,3 Mannosidase GE1001 1,000 units No 7.0 Cleaves terminal α1,3-linked mannose

Glycoproteins digested with Lectenz Bio PNGase F or PNGase F-II. A. PNGase F is commonly used to cleave N-glycans from mammalian proteins with or without α1,6-core fucosylation, such as bovine ribonuclease B (RNase B). B. PNGase F-II can also cleave RNase B, as well as α1,3-core fucosylated N-glycans from insect or plant proteins, such as horseradish peroxidase (HRP).