PNGase F Enzyme by Lectenz Bio: Efficient Deglycosylation
Price range: $190.00 through $645.00
| Product | SKU | Quantity | Price |
|---|---|---|---|
| PNGase F | GE0101 | 4,000 Units | $190 |
| N-GlyFind™ Kit w/ PNGase F | NK0102 | 1 Kit | $520 |
| N-GlyFind™ Biotinylated Kit w/ PNGase F | NK0102B | 1 KIt | $575 |
| N-GlyFind™ SureLight® 488 Kit w/ PNGase F | NK0102F | 1 KIt | $645 |
| Custom Order | Inquire |
This product is recombinant PNGase F (Peptide: N-Glycosidase F, EC #3.5.1.52, CAS #83534-39-8), cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, except those containing an α1,3-linked core fucose, from glycoproteins and glycopeptides. PNGase F enzyme does not contain any detectable activities of proteases or other glycosidases. Unit Definition: One unit is defined as the amount of PNGase F required to deglycosylate 1 nanomole (15 μg) of denatured RNase B in 1 h at 37°C in 25 μL 1X Reaction Buffer (20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).
N-GlyFind™ Kits w/ PNGase F (NK0102, NK0102B, and NK0102F) contain N-GlyFind™ Core-Specific Reagent for detection of ManGlcNAc2-Asn within the N-glycan core structure commonly found in glycoproteins.
Supplementary Information
Product reconstitution: Dissolve the lyophilized product in 100 μL ultrapure water to make a 40,000 units/mL solution in 1X Reaction Buffer. Once reconstituted, store at 4°C for up to 10 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
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