PNGase F

PNGase F

$150.00$600.00

Product SKU UnitsPrice
PNGase FGE0101-4KU 4,000$150
PNGase FGE0101-20KU 20,000$600
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SKU: GE0101 Category: Tag: Product ID: 141798

Description

This product is recombinant PNGase F (Peptide:N-Glycosidase F, EC #3.5.1.52, CAS #83534-39-8), cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, except those containing an α1,3-linked core fucose, from glycoproteins and glycopeptides.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of PNGase F required to deglycosylate 1 nanomole (15 μg) of denatured RNase B in 1 h at 37°C in 25 μL 1X Reaction Buffer (20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).

Additional information

Weight 4.5 oz
Dimensions 285.75 × 222.25 × 111.125 mm
PNGase F

4,000 U, 20,000 U

Supplementary Information

Dissolve the lyophilized product in 100 μL ultrapure water to make a 40,000 units/mL (Cat#GE0101-4KU) or a 200,000 units/mL (Cat #GE0101-20KU) solution in 1X Reaction Buffer. Once reconstituted, store at 4°C for up to 10 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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Product Information Sheet

PNGase F Information Sheet

SDS

PNGase F SDS