Beta-N-Acetylhexosaminidase

$75.00

Product	SKU	Units	Price
Beta-N-Acetylhexosaminidase	GE1101	500	$75
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SKU: GE1101 Category: Glycan Processing Tag: Enzyme

Description
Supplementary Information
Product Information Sheet
SDS

This product is recombinant β-N-Acetylhexosaminidase (glycosyl hydrolase family GH20; E.C. 3.2.1.52), cloned from Streptococcus pneumoniae and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

This product catalyzes the hydrolysis of the non-reducing terminal N-Acetylglucosamine (GlcNAc) and N-Acetylgalactosamine (GalNAc) from oligosaccharides and glycoprotein substrates.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-GlcNAc) in 1 min at 37°C in 100 μL. 1X Reaction Buffer 4 (50 mM Tris-HCl, 100 mM NaCl, pH 7.5).

Supplementary Information

Product reconstitution: Dissolve the lyophilized product in 100 μL molecular grade water to make a 5,000 units/mL (Cat #GE1101) solution in enzyme storage buffer (50 mM Tris-HCl, 100 mM NaCl, pH 7.5). Once reconstituted, store at 4°C for up to 7 days or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.