This product is recombinant PNGase F-II, cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, with or without core fucose, from glycoproteins and glycopeptides. Unlike PNGase F, which cannot cleave N-glycans with α1,3-linked core fucose, PNGase F-II can cleave N-glycans with α1,6- or α1,3-linked core fucose.
This product does not contain any detectable activities of proteases or other glycosidases.
Unit Definition: One unit is defined as the amount of PNGase F-II required to deglycosylate 1 nanomole (15 μg) of denatured RNase B or 0.1 nanomole (4.5 μg) of horse radish peroxidase (HRP) in 2 h at 37°C in 25 μL 1X Reaction Buffer (50 mM Bis-Tris, 100 mM NaCl, pH 7.0).