FasTEV™ Protease

FasTEV™ Protease

$100.00

Product SKU UnitsPrice
FasTEVTM ProteaseGE0501-1KU 1,000$100
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SKU: GE0501 Category: Tag: Product ID: 184225

Description

Tobacco Etch Virus (TEV) Protease (EC#3.4.22.44) specifically cleaves the peptide bond between a glutamine and a glycine or serine of the consensus sequence, ENLYFQ↓(G/S).

FasTEVTM is an engineered version of the enzyme with multiple mutations that enhance its stability and catalytic activity. It is recombinantly expressed in Escherichia coli with an N-terminal 8xHis tag. This product does not contain any detectable activities of nonspecific proteases.

Unit Definition: One unit is defined as the amount of FasTEVTM required to cleave 90% of 0.1 nanomole (5.4 μg) of a fusion protein substrate in 1 h at 30°C in 20 μL 1X Reaction Buffer 5 (50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).

Additional information

Weight 4.5 oz
Dimensions 285.75 × 222.25 × 111.125 mm
FasTEV Protease

1,000 U

Supplementary Information

Dissolve the lyophilized product in 100 μL molecular grade water to make a 10,000 units/mL (GE0501-1KU) solution in 1X Reaction Buffer 5 (50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5). Once reconstituted, FastTEVTM can be stored at 4°C for up to 2 weeks or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

Product Information Sheet

FasTEVTM Information Sheet

SDS

FasTEVTM SDS