This product is a recombinant α1,3 mannosidase (glycosyl hydrolase family GH38, EC#22.214.171.124) cloned from Streptococcus pyogenes and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.
This product catalyzes the hydrolysis of terminal α1,3-linked mannose (Man) residues from oligosaccharides and glycoprotein substrates.
This product does not contain any detectable activities of proteases or other glycosidases.
Unit definition: One unit is defined as the amount of α1,3 Mannosidase required to catalyze the release of 1 nmole 4-methylumbelliferone (4MU) from 4-methylumbelliferyl α-D-mannopyranoside (4MU-Man) in 30 min at 37°C in 50 μL 1X Reaction Buffer 2 (50 mM Bis-Tris, 100mM NaCl, pH 7.0), plus 0.2 mM ZnSO4 and 1 mg/mL BSA.