Alpha 1,3 Mannosidase: Precision Glycan Editing | Lectenz Bio
$150.00
| Product | SKU | Units | Price |
|---|---|---|---|
| α1,3 Mannosidase | GE1001 | 1,000 | $150 |
| Custom Order | Inquire |
This product is a recombinant α1,3 mannosidase (glycosyl hydrolase family GH38, EC#3.2.1.24) cloned from Streptococcus pyogenes and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.
Our alpha mannosidase catalyzes the hydrolysis of terminal α1,3-linked mannose (Man) residues from oligosaccharides and glycoprotein substrates.
This product does not contain any detectable activities of proteases or other glycosidases.
Unit definition: One unit is defined as the amount of α1,3 Mannosidase required to catalyze the release of 1 nmole 4-methylumbelliferone (4MU) from 4-methylumbelliferyl α-D-mannopyranoside (4MU-Man) in 30 min at 37°C in 50 μL 1X Reaction Buffer 2 (50 mM Bis-Tris, 100mM NaCl, pH 7.0), plus 0.2 mM ZnSO4 and 1 mg/mL BSA.
Supplementary Information
Form and Storage: The product is supplied as a 10,000 units/mL (Cat #GE1001) solution in enzyme storage buffer (20 mM Tris-HCl, 100 mM NaCl, pH 7.5). Upon arrival, store at 4°C for up to 7 days or -80°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
