Alpha 1,3 Mannosidase

Alpha 1,3 Mannosidase


α1,3 MannosidaseGE10011,000$150
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SKU: GE1001 Category: Tag: Product ID: 258369


This product is a recombinant α1,3 mannosidase (glycosyl hydrolase family GH38, EC# cloned from Streptococcus pyogenes and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

This product catalyzes the hydrolysis of terminal α1,3-linked mannose (Man) residues from oligosaccharides and glycoprotein substrates.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit definition: One unit is defined as the amount of α1,3 Mannosidase required to catalyze the release of 1 nmole 4-methylumbelliferone (4MU) from 4-methylumbelliferyl α-D-mannopyranoside (4MU-Man) in 30 min at 37°C in 50 μL 1X Reaction Buffer 2 (50 mM Bis-Tris, 100mM NaCl, pH 7.0), plus 0.2 mM ZnSO4 and 1 mg/mL BSA.

Additional information

Weight 4.5 oz
Dimensions 285.75 × 222.25 × 111.125 mm

Supplementary Information

Form and Storage: The product is supplied as a 10,000 units/mL (Cat #GE1001) solution in enzyme storage buffer (20 mM Tris-HCl, 100 mM NaCl, pH 7.5). Upon arrival, store at 4°C for up to 7 days or -80°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

Product Information Sheet

α1,3 Mannosidase Information Sheet


α1,3 Mannosidase SDS