SiaFind™ α2,3-Specific Lectenz® is a sialic acid affinity reagent for the detection, separation, and enrichment of sialoglycans terminating in Neu5Acα2,3Gal commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards α2,3 linked sialic acids on glycans. However, it does not bind effectively to branched sialylated epitopes such as sialyl Lewis A/X. This reagent can be employed as a capture reagent in a variety of applications.
SiaRich™ α2,3-Specific Resin (Cat #SR2301) is an agarose resin coupled with the SiaFind™ α2,3-Specific Lectenz® and is designed for the rapid and specific enrichment of sialoglycoconjugates terminating in Neu5Acα2,3Gal from a complex sample. Specifically bound α2,3-sialylated glycoconjugates are non-competitively eluted from the resin for downstream analysis such as mass spectrometry, ELISA, and Western blotting.
Supplementary Information
Form and Storage
The SiaRich™ α2,3-Specific Resin is supplied as a 50% slurry in Tris Buffered Saline ready for affinity chromatography. The kit includes 2 bottles of 5X Binding Buffer 2 (Cat #BA0102) for equilibration, binding, washing, and storage.
