Supplementary Information

Each SiaFind Lectenz^® reagents has a molecular mass of about 77 kD and works as a monomer in a simple buffer without bivalent metal ions.  They are His₆-tagged proteins and an anti-His₆ antibody or its conjugated forms may be used as a 2° antibody for detection.

In addition to the His₆-tag, the biotinylated SiaFind Lectenz^® reagents can be detected using a streptavidin conjugate.

Sia Lectenz^® reagents are sensitive to salt. Titration of NaCl concentration in binding buffer may be performed. Increasing salt concentration reduces both non-specific and specific binding capacity. The recommended low salt binding buffer is 10 mM EPPS, 10 mM NaCl, pH 7.5.

Form and Storage

The SiaFind reagents are supplied in Sia Lectenz^® Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5).  Concentration is determined by NanoDrop spectrophotometry using E^1%12.7.  They are most stable frozen at -80°C. At 4°C, they are stable for about 1 week. Avoid repeated freeze-thaw. However, aliquoting and refreezing once will not significantly reduce binding activity.

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Easy and specific detection using Western blot reagents.