Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.
SiaFindTM α2,3-Specific Lectenz® Kits (Cat #SK2301 and SK2301B) contain a sialic acid affinity reagent for the detection, separation, or enrichment of sialoglycans terminating in Neu5Acα2,3Gal commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards α2,3 linked sialic acids on glycans. However, it does not bind effectively to branched sialylated epitopes such as sialyl Lewis A/X. Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use.
Each SiaFindTM Lectenz® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. They are 6xHis-tagged proteins and an anti-6xHis antibody or, in the case of the biotinylated version, a streptavidin conjugate can be used for detection.
Form and Storage
The SiaFindTM reagents are supplied lyophilized in 1X Sia Lectenz® Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 12.7. Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X Sia Lectenz® Buffer to 200 mL water and mix by inversion. All Sia Lectenz® Buffers may be stored at 4 to 25°C.