Supplementary Information
Form and Storage
The SiaRich™ affinity column is prepacked and FPLC-ready. It is supplied in SiaFind™ Binding Buffer 3 (SBB3; 25 mM EPPS, 110 mM NaCl, pH 7.5). It is stable at 4°C for three months from the date of production. Never freeze the column.
All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X buffer to 200 mL water and mix by inversion. All buffers may be stored at 4 to 25°C.