Supplementary Information

Each SiaFind^TM Lectenz^® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. They are 6xHis-tagged proteins and an anti-6xHis antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection.

Form and Storage

The SiaFind^TM reagents are supplied lyophilized in 1X Sia Lectenz^® Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E^1% 12.7. Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X Sia Lectenz^® Buffer to 200 mL water and mix by inversion.  All Sia Lectenz^® Buffers may be stored at 4 to 25°C.

Western Blot