Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages (see supplementary information) over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.
SiaFind Pan-Specific Lectenz®Kits (Cat # SK0501 and SK0501B) contain a sialic acid affinity reagent for the detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards sialoglycans in a linkage independent manner (pan-specific). Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use and saving you valuable bench time making buffers.
200 × 200 × 200 mm
1mg, 1mg biotinylated
Each SiaFind Lectenz® reagent has a molecular mass of about 77 kD and works as a monomer in a simple buffer without bivalent metal ions. They are His6-tagged proteins and an anti-His6 antibody or its conjugated forms may be used as a 2° antibody for detection.
In addition to the His6-tag, the biotinylated SiaFind Lectenz® reagents can be detected using a streptavidin conjugate.
Sia Lectenz® reagents are sensitive to salt. Titration of NaCl concentration in binding buffer may be performed. Increasing salt concentration reduces both non-specific and specific binding capacity. The recommended low salt binding buffer is 10 mM EPPS, 10 mM NaCl, pH 7.5.
Form and Storage
The SiaFind reagents are supplied in Sia Lectenz® Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5). Concentration is determined by NanoDrop spectrophotometry using E1%12.7. They are most stable frozen at -80°C. At 4°C, they are stable for about 1 week. Avoid repeated freeze-thaw. However, aliquoting and refreezing once will not significantly reduce binding activity.