Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.
SiaFind™ Pan-Specific Lectenz® 2.0 Kits (Cat #SK0502 and SK0502B) contain a high-performance sialic acid affinity reagent engineered from the original SiaFind™ Pan-Specific Lectenz® (Cat #SK0501 and SK0501B) for the robust detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards sialoglycans in a linkage independent manner (pan-specific). Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use.
SiaFind™ Pan-Specific Lectenz® 2.0 has a molecular mass of about 57 kD and works as a monomer without bivalent metal ions. It is 8xHis-tagged at its N-terminus, and an anti-polyhistidine antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection. The 8xHis tag may be removed by FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.
The original SiaFind™ Pan-Specific Lectenz® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. It is 6xHis-tagged at its N-terminus, and an anti-polyhistidine antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection.
Form and Storage
The SiaFind™ Pan-Specific Lectenz® 2.0 are supplied lyophilized in a storage buffer (50 mM EPPS, 200 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular grade water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 10.4.
The original SiaFind™ Pan-Specific Lectenz® are supplied lyophilized in a storage buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular grade water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 12.7.
Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X buffer to 200 mL water and mix by inversion. All buffers may be stored at 4 to 25°C.
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