SiaFind Pan-Specific Lectenz® Kit

Price range: $375.00 through $500.00

Product	SKU	MG	Price
SiaFind™ Pan-Specific Lectenz® 2.0 Kit	SK0502	1	$375
SiaFind™ Pan-Specific Lectenz® 2.0 Biotinylated Kit	SK0502B	1	$425
SiaFind™ Pan-Specific Lectenz® Kit	SK0501	1	$375
SiaFind™ Pan-Specific Lectenz® Biotinylated Kit	SK0501B	1	$425
SiaFind™ Pan-Specific Lectenz® SureLight® 488 Kit (2 week lead time)	SK0501F	1	$500
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SiaFind™ Pan-Specific Lectenz®

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SKU: SK0502 Category: Glycan Detection Tag: Sialic acid

Description
Supplementary Information
Product Information Sheet
SDS

Lectenz^® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.

SiaFind™ Pan-Specific Lectenz^® 2.0 Kits (Cat #SK0502 and SK0502B) contain a high-performance sialic acid affinity reagent engineered from the original SiaFind™ Pan-Specific Lectenz^® (Cat #SK0501 and SK0501B) for the robust detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards sialoglycans in a linkage independent manner (pan-specific). Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use.

Supplementary Information

SiaFind™ Pan-Specific Lectenz^® 2.0 has a molecular mass of about 57 kD and works as a monomer without bivalent metal ions. It is 8xHis-tagged at its N-terminus, and an anti-polyhistidine antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection. The 8xHis tag may be removed by FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

The original SiaFind™ Pan-Specific Lectenz^® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. It is 6xHis-tagged at its N-terminus, and an anti-polyhistidine antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection. The fluorescent version can be directly detected.

Form and Storage

The SiaFind™ Pan-Specific Lectenz^® 2.0 are supplied lyophilized in a storage buffer (50 mM EPPS, 200 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular grade water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 10.4.

The original SiaFind™ Pan-Specific Lectenz^® (native and biotinylated) are supplied lyophilized in a storage buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular grade water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 12.7.

The SiaFind™ Pan-Specific Lectenz^® SureLight^® 488 reagent is supplied as a 10 mg/mL solution in a storage buffer (50 mM EPPS, 100 mM NaCl, pH 7.5). Concentration is determined by spectrophotometry using E1% 12.7. The actual absorbance at 280 nm is calculated by measuring the observed absorbance at 280 nm and subtracting the absorbance at 493 nm multiplied by a correction factor of 0.11; A280_actual = A280_observed – (A493 x 0.11). Store in dark tubes, and perform experiments protected from light.

Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X buffer to 200 mL water and mix by inversion. All buffers may be stored at 4 to 25°C.