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SiaFind™ Pan-Specific Lectenz® Kit

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SiaFind™ Pan-Specific Lectenz® Kit

$275.00 – $325.00

Product SKU MGPrice
SiaFind™ Pan-Specific Lectenz® KitSK0501 1$275
SiaFind™ Pan-Specific Lectenz®, Biotinylated KitSK0501B 1$325
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SKU: SK0501 Category: Tags: , Product ID: 140354

Description

Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.

SiaFind™ Pan-Specific Lectenz® Kits (Cat #SK0501 and SK0501B) contain a sialic acid affinity reagent for the detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards sialoglycans in a linkage independent manner (pan-specific). Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use.

Additional information

Weight 4.5 oz
Dimensions 285.75 × 222.25 × 111.125 mm
SiaFind™ Pan-Specific Lectenz®

1 mg, 1 mg biotinylated

Supplementary Information

Each SiaFind™ Lectenz® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. They are 6xHis-tagged proteins and an anti-6xHis antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection.

Form and Storage

The SiaFind™ Lectenz® are supplied lyophilized in a storage buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular grade water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 12.7. Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X buffer to 200 mL water and mix by inversion. All buffers may be stored at 4 to 25°C.

Western Blot

Product Information Sheet

SiaFind™ Pan-Specific Lectenz Information Sheet

SDS

SiaFind™ Pan-Specific Lectenz SDS

Citations

1. Dabelsteen S, Pallesen EMH, Marinova IN, Nielsen MI, Adamopoulou M, Rømer TB, Levann A, Andersen MM, Ye Z, Thein D, Bennett EP, Büll C, Moons SJ, Boltje T, Clausen H, Vakhrushev SY, Bagdonaite I, Wandall HH. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Dev Cell. 2020 Sep 14;54(5):669-684.e7. doi: 10.1016/j.devcel.2020.06.039. Epub 2020 Jul 24. PMID: 32710848; PMCID: PMC7497784.

2. Büll C, Nason R, Sun L, Van Coillie J, Madriz Sørensen D, Moons SJ, Yang Z, Arbitman S, Fernandes SM, Furukawa S, McBride R, Nycholat CM, Adema GJ, Paulson JC, Schnaar RL, Boltje TJ, Clausen H, Narimatsu Y. Probing the binding specificities of human Siglecs by cell-based glycan arrays. Proc Natl Acad Sci U S A. 2021 Apr 27;118(17):e2026102118. doi: 10.1073/pnas.2026102118. PMID: 33893239; PMCID: PMC8092401.

3. Nason R, Büll C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, Sørensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, de Haan CAM, Vincentelli R, Henrissat B, Vakhrushev SY, Clausen H, Narimatsu Y. Display of the human mucinome with defined O-glycans by gene engineered cells. Nat Commun. 2021 Jul 1;12(1):4070. doi: 10.1038/s41467-021-24366-4. PMID: 34210959; PMCID: PMC8249670.

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