Alpha 2,3 Sialidase

Alpha 2,3 Sialidase

$225.00

Product SKU UnitsPrice
α2,3 Sialidase GE0302-5KU 5,000$225
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SKU: GE0302-5KU Category: Tag: Product ID: 141803

Description

This product is a recombinant neuraminidase (exo-α-sialidase, EC #3.2.1.18, CAS #9001-67-6), cloned from Streptococcus pneumoniae and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEVTM (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

This product does not contain any detectable activities or proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of α2,3 Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 10 min at 37°C in 100 μL 1X Reaction Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5).

Additional information

Weight 4.5 oz
Dimensions 285.75 × 222.25 × 111.125 mm

Supplementary Information

Product reconstitution: Dissolve the lyophilized product in 100 μL of ultrapure water to make a 50,000 units/ml (Cat #GE0302-5KU) solution in 1X Reaction Buffer. Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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Product Information Sheet

α2,3 Sialidase Information Sheet

SDS

α2,3 Sialidase SDS