This product is a recombinant neuraminidase (exo-α-sialidase, EC #22.214.171.124, CAS #9001-67-6), cloned from Streptococcus pneumoniae and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEVTM (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.
This product preferentially releases terminal α2,3-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins.
This product does not contain any detectable activities or proteases or other glycosidases.
Unit Definition: One unit is defined as the amount of α2,3 Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 1 min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM EPPS, 100 mM NaCl, pH 7.5).