Alpha 2,3 Sialidase

Alpha 2,3 Sialidase

$225.00

Product SKU UnitsPrice
α2,3 Sialidase GE0301-5KU 5,000$225
α2,3 SialidaseGE0301-50KU50,000Inquire
Custom OrderInquire
SKU: GE0301-5KU Category: Tag: Product ID: 141803

Description

This product is a recombinant neuraminidase (exo-α-sialidase, EC #3.2.1.18, CAS #9001-67-6), cloned from Streptococcus pneumoniae and expressed in Escherichia coli. It preferentially releases terminal α2,3-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins.

This product does not contain any detectable activities or proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of α2,3 Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 10 min at 37°C in a total reaction volume of 100 μL, pH 7.5.

Additional information

Weight 4.5 oz
Dimensions 200 × 200 × 200 mm

Supplementary Information

Product reconstitution: Dissolve the lyophilized product in 100 μL of molecular grade water to make a 50,000 units/ml (Cat # GE0301) or a 500,000 units/mL (Cat # GE0301L) solution in 1X Reaction Buffer(50mM EPPS, 100mM NaCl, 1mM EDTA, pH 7.5). Once reconstituted, store at 4°C for up to 5 days or -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.

Product Information Sheet

α2,3 Sialidase Information Sheet

SDS

α2,3 Sialidase SDS