GlycoSense™ is a multiplex assay for the detection of glycosylation patterns on glycoproteins using a two-laser flow cytometer. The GlycoSense™ array consists of lectins conjugated to multiplex microspheres (beads) that bind terminal monosaccharides on a glycoprotein to quickly profile the glycan features present. This simplified “GlycoPrint” does not give a full glycosylation profile or structural information, however, is capable of detecting changes in several key glycan features. The microspheres in this array have been evaluated to bind sialic acid, galactose and N-acetylglucosamine (GlcNAc) on N-linked glycans.
285.75 × 222.25 × 111.125 mm
GlycoSense™ Array Kit
The GlycoSense™ beads are excitable with a 633/640 nm laser and can be measured in the APC or APC-Cy7 fluorescence channels on conventional flow cytometers. The GlycoSense™ assay requires either the direct labeling of the glycoprotein or of the glycoprotein detecting reagent, such as a binding partner or Fab fragment, with a 488 nm excitable fluorophore (Alexa Fluor® 488, DyLight® 488, FITC, SureLight™ 488). A fluorescent protein (e.g., GFP)-tagged recombinant glycoprotein can also be directly detected. Antibodies are not recommended for secondary detection of glycoproteins, as antibody glycosylation may interfere with the assay.
1. Saunders MJ, Woods RJ, Yang L. Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering. Sci Rep. 2023 Jan 11;13(1):567. doi: 10.1038/s41598-023-27634-z. PMID: 36631484; PMCID: PMC9834283.