GlycoSense Array Kit
$600.00
| Product | SKU | Assays | Price |
|---|---|---|---|
| GlycoSense™ Array Kit (2 week lead time) | GK0101-50UL | 50 | $600 |
| Custom Order | Inquire |
GlycoSense™ is a multiplex assay for detecting glycosylation patterns on glycoproteins using a standard two-laser flow cytometer. The GlycoSense™ array consists of proteins conjugated to spectrally distinct microspheres (beads), each exhibiting a unique red fluorescence intensity for population identification. The GlycoSense™ beads include a BSA-conjugated background control, along with lectin-conjugated beads specific for detection of α2,6-linked sialic acid (SNA), α2,3-linked sialic acid (MAL I), β1,4-linked galactose (ECA), and N-acetylglucosamine (GSL II), enabling rapid profiling of key terminal features on N-linked glycans. This simplified “GlycoPrint” does not provide a complete glycosylation profile or structural information, but is well-suited for detecting relative changes in select glycosylation attributes.
Supplementary Information
The GlycoSense™ beads are excitable with a 633/640 nm laser and can be measured in the APC, APC-Cy7, or FL4 fluorescence channels on conventional flow cytometers. The GlycoSense™ assay requires either direct labeling of the glycoprotein of interest or of the glycoprotein detecting reagent, such as a binding partner or Fab fragment, with a 488 nm excitable fluorophore (e.g., Alexa Fluor® 488, DyLight® 488, FITC, SureLight® 488). A fluorescent protein (e.g., GFP-tagged recombinant glycoprotein) can also be directly detected. Intact antibodies are not recommended for secondary detection of glycoproteins, as antibody glycosylation may interfere with the assay.