PNGase F Enzyme by Lectenz Bio: Efficient Deglycosylation
$150.00
| Product | SKU | Units | Price |
|---|---|---|---|
| PNGase F | GE0101 | 4,000 | $150 |
| Custom Order | Inquire |
This product is recombinant PNGase F (Peptide:N-Glycosidase F, EC #3.5.1.52, CAS #83534-39-8), cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, except those containing an α1,3-linked core fucose, from glycoproteins and glycopeptides.
PNGase F enzyme does not contain any detectable activities of proteases or other glycosidases.
Unit Definition: One unit is defined as the amount of PNGase F required to deglycosylate 1 nanomole (15 μg) of denatured RNase B in 1 h at 37°C in 25 μL 1X Reaction Buffer (20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).
Supplementary Information
Product reconstitution: Dissolve the lyophilized product in 100 μL ultrapure water to make a 40,000 units/mL solution in 1X Reaction Buffer. Once reconstituted, store at 4°C for up to 10 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
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