N-GlyRich Core-Specific Resin Kit
$550.00
| Product | SKU | ML | Price |
|---|---|---|---|
| N-GlyRich™ Core-Specific Reagent Kit | NR0101 | 2 | $550 |
| Custom Order | Inquire |
N-GlyFind™ Core-Specific Reagent is a recombinant protein engineered from Mus musculus Fbs1 (Fbxo2), a component of the E3 ubiquitin ligase complex. This highly purified affinity reagent is designed for sensitive, robust, and specific detection of ManGlcNAc2-Asn within the N-glycan core structure commonly found in glycoproteins.
N-GlyRich™ Core-Specific Resin (Cat #NR0101) is an agarose resin coupled with the N-GlyFind™ Core-Specific Reagent and is designed for the rapid and specific enrichment of N-glycoproteins and N-glycopeptides from a complex sample. Specifically bound N-glycoconjugates are non-competitively eluted from the resin for downstream analysis such as mass spectrometry, ELISA, and Western blotting.
Supplementary Information
Form and Storage
The N-GlyFind™ Core-Specific Resin is supplied as a 50% suspension in Tris Buffered Saline ready for affinity chromatography. The kit includes 5X Tris Buffered Saline (Cat #BA0104) for binding, washing, and storage; 5X Elution Buffer 2 (Cat #BA0202); and endoproteinase GluC-digested Ribonuclease B (Cat #GC0201) as an analyte control.
All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X buffer to 200 mL water and mix by inversion. All buffers may be stored at 4 to 25°C.
The Ribonuclease B peptides should be reconstituted in 50 μL molecular grade water to make a solution of 1.0 mg/mL in GluC digestion buffer (0.1 M NaCl, 50 mM Tris-HCl, 0.5 mM Glu-Glu, pH 8.0). An amount of 10 μg may be used as a control analyte. Non-glycosylated peptides should flow through, while N-glycopeptides should bind and be subsequently eluted.
