This product is a recombinant neuraminidase (exo-α-sialidase; glycosyl hydrolase family GH33, EC #3.2.1.18), cloned from Arthrobacter ureafaciens and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity. This product catalyzes the hydrolysis of terminal α-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins.
This product catalyzes the hydrolysis of terminal α-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins.
This product does not contain any detectable activities or proteases or other glycosidases.
Unit definition: One unit is defined as the amount of Pan Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 1 min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM Tris-HCl, 100 mM NaCl, pH 7.5).
Supplementary Information
Product reconstitution: Dissolve the lyophilized product in 100 μL of molecular grade water to make a 50,000 units/ml (Cat #GE0701) solution in enzyme storage buffer (50 mM EPPS, 100 mM NaCl, pH 7.5). Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.
