SiaRich™ Pan-Specific Sialic Acid Affinity Column

$250.00$1,000.00

Product SKU MLPrice
SiaRich™ Pan-Specific Sialic Acid Affinity ColumnSC0005-1ML 1$250
SiaRich™ Pan-Specific Sialic Acid Affinity ColumnSC0005-5X1mL 1 x 5$1000
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SKU: SC0005 Category: Tags: , , Product ID: 3233

Description

Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution.  The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure.  They have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates, making glycoscience simple.

The Pan-Specific Lectenz® is a sialic acid affinity reagent for the detection, separation and enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo or polysaccharides).  This reagent can be employed as a capture reagent in a variety of applications.

The SiaRich Pan-Specific Lectenz®Affinity Columns (Cat # SC0005) are pre-packed ligand-coupled columns for rapid enrichment and purification of all sialoglycoconjugates in a linkage independent manner (pan-specific) in a process called Lectenz®Affinity Chromatography (LAC).  Specifically bound sialylated glycoproteins or other biomolecules may be eluted non-competitively from the column using a simple high-salt buffer.

Additional information

SiaRich PS Affinity column

1mL, 5 x 1mL

Supplementary Information

Form and Storage

The SiaRich affinity columns are prepacked and FPLC-ready.  They are supplied in LAC Column Storage Buffer (LCSB; 50 mM EPPS, 100 mM NaCl, pH 7.5).  They are stable at 4°C for three months.  Never freeze the columns.

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Pan-Specific Sia-PS5 separates sialoglycoprotein. Sia-PS5 (2 mg) was chemically coupled to a 1-mL Sepharose column, followed by loading of an analyte. (A)  Flow-through of non-sialylated glycoprotein horseradish peroxidase (blue line) and retention of highly sialylated glycoprotein fetuin(orange line) in overlaid chromatograms. (B) Chromatogram showing separation of an equal mixture of BSA (non-glycosylated protein) and fetuin. (C) Separation of an equal mixture of de-sialylated fetuin (asialofetuin) andfetuin.

 

Product Datasheet

SiaRich Pan-Specific Column Datasheet

SDS

SiaRich Column SDS