Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure. They have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates, making glycoscience simple.
The Pan-Specific Lectenz® is a sialic acid affinity reagent for the detection, separation and enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo or polysaccharides). This reagent can be employed as a capture reagent in a variety of applications.
The SiaRich Pan-Specific Lectenz®Affinity Columns (Cat # SC0005) are pre-packed ligand-coupled columns for rapid enrichment and purification of all sialoglycoconjugates in a linkage independent manner (pan-specific) in a process called Lectenz®Affinity Chromatography (LAC). Specifically bound sialylated glycoproteins or other biomolecules may be eluted non-competitively from the column using a simple high-salt buffer.