SiaFind™ alpha 2,3-Specific Lectenz®

$150.00$500.00

Product SKU MGPrice
SiaFind™ α2,3-SpecificSP2301-1MG 1$150
SiaFind™ α2,3-SpecificSP2301-5MG 5$500
SiaFind™ α2,3-Specific - BiotinylatedSP2301B-1MG 1$200
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SKU: SP2301 Category: Tag: Product ID: 3298

Description

Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution.  The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure.  They have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates, making glycoscience simple.

SiaFind α2,3-Specific Lectenz® (Cat # SP2301 and SP2301B) is a sialic acid affinity reagent for the detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides).  It has high affinity and specificity towards α2,3 linked sialic acids on glycans.

Additional information

SiaFind α2,3-Linkage

1mg, 5mg, 1mg biotinylated

Supplementary Information

Each SiaFind Lectenz® has a molecular mass of about 77 kD and works as a monomer in a simple buffer without bivalent metal ions.  They are His6-tagged proteins and an anti-Hisantibody or its conjugated forms may be used as a 2° antibody for detection.

In addition to the His6-tag, the biotinylated SiaFind Lectenz® reagents can be detected using a streptavidin conjugate.

Sia Lectenz® reagents are sensitive to salt. Titration of NaCl concentration in binding buffer may be performed. Increasing salt concentration reduces both non-specific and specific binding capacity. The recommended low salt binding buffer is 10 mM EPPS, 10 mM NaCl, pH 7.5.

Form and Storage

The SiaFind reagents are supplied in Sia Lectenz®Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5).  Concentration is determined by NanoDrop spectrophotometry using E1%12.7.  They are most stable frozen at -80°C. At 4°C, they are stable for about 1 week. Avoid repeated freeze-thaw. However, aliquoting and refreezing once will not significantly reduce binding activity.

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Easy and specific detection using Western blot reagents.

 

 

 

 

 

 

Product Datasheet

SiaFind α2,3-Specific Lectenz Datasheet

SDS

SiaFind Lectenz SDS