Description

This product is recombinant PNGase F (Peptide:N-Glycosidase F, EC #3.5.1.52, CAS #83534-39-8), cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, except those containing an α1,3-linked core fucose, from glycoproteins and glycopeptides.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of PNGase F required to deglycosylate 1 nanomole (15 μg) of denatured RNase B in 1 h at 37°C in 25 μL 1X Reaction Buffer (20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).