Testing Product

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The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates.

Product ID: 1753

Description

Pierce™ Glycoprotein Isolation Kit, WGA

Catalog number: 89805

Thermo Scientific™  Related applications: Protein Purification & Isolation

Catalog number
Unit size
Price (USD) Qty
89805
10 reactions
335.00
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Overview

Manuals

MSDS

COA

FAQs

Citations & references

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Description

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing N-Acetyl glucosamine (GlcNAc) groups or sialic acid. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
Fast—glycoprotein purification in less than an hour
Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
Robust—lectin does not leach from resin when processing sample
Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, ConA

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Column Type: Affinity, Agarose Resin
Format: Kit, Microcentrifuge, Spin Column
Immobilized Phase: Lectin
Product Line: Pierce™
Product Size: 10 reactions

Contents & storage

Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 µL (1 to 1.5 mg total protein) each
• WGA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubesStore at 4°C.

Frequently asked questions (FAQs)

Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?

What is the difference between your Pierce™ Glycoprotein Isolation Kit, ConA and WGA?

Citations & references

Cooper CA, Harrison MJ, Wilkins MR, Packer NH
Nucleic Acids Res 2001; (29):1 332-335

Ghosh D, Krokhin O, Antonovici M, Ens W, Standing KG, Beavis RC, Wilkins JA
J Proteome Res ; (3):4 841-850

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2 reviews for Testing Product

  1. lectenz® Bio

    This is a test

  2. Rated 3 out of 5

    Foodormunerne

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